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Frequently Asked Questions
Here are the most common questions about reagent in peptide cleavage.
Introduction to Cleavage Techniques
10 May 2023—As I mentioned in my first answer, justevaporate cleavage reagents(TFA/TIS/Water) and your peptide will precipitate. Do not use diethyl ether.
Peptide Cleavage from Resin Protocol - CDN
Cleavagefrom Resin. Typically, acid-liable groups are used to anchor the C terminus of thepeptideto the resin, e.g., 2-chlorotrityl chloride.